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ISSN : 2288-1115(Print)
ISSN : 2288-1123(Online)
Korean Journal of Ecology and Environment Vol.45 No.2 pp.218-231

폐광산 지역의 유출수에 대한 이∙화학적 수질특성 및 Enclosure 어류 노출시험 평가

안광국*, 배대열, 한정호
충남대학교 생명과학과

Physico-chemical Characteristics and In situ Fish Enclosure Bioassays on Wastewater Outflow in Abandoned Mine Watershed

Kwang-Guk An*, Dae-Yeul Bae, Jeong-Ho Han
Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University
(Manuscript received 28 March 2012, Revised 8 May 2012, Revision accepted 30 May 2012)


The objectives of this study were to evaluate the physico-chemical water quality,trophic and tolerance guilds in the control (Co) and impacted streams of the abandonedmine, along with the ecological health, using a multimetric health model andphysical habitat conditions of Qualitative Habitat Evaluation Index (QHEI), duringthe period of three years, 2005~2007. Also, eco-toxicity (EEt) enclosure tests wereconducted to examine the toxic effects on the outflows from the mine wastewater,using the sentinel species of Rhynchocypris oxycephalus, and we compared the biologicalresponses of the control (Co) and treatment (T) to the effluents through a NecropybasedHealth Assessment Index (Nb-HAI). Tissue impact analysis of the spleen, kidney,gill, liver, eyes, and fins were conducted in the controlled enclosure experiments (10individuals). According to the comparisons of the control (Co) vs. the treatment (T) inphysicochemical water quality, outflows from the abandoned mine resulted in lowpH of 3.2, strong acid wastewater, high ionic concentrations, based on an electricalconductivity, and high total dissolved solid (TDS). Physical habitat assessments, basedon Qualitative Habitat Evaluation Index (QHEI) did not show any statistical differences(p>0.05) in the sampling sites, whereas, the Mm-EH model values in a multimetricecological health (Mm-EH) model of the Index of Biological Integrity (IBI), usingfish assemblages, were 16~20 (fair condition) in the control and all zero (0, poor condition)in the impacted sites of mine wastewater. In addition, in enclosure eco-toxicity(EEt) tests, the model values of Nb-HAI ranged between 0 and 3 in the controls duringthe three years, indicating an excellent~good condition (Ex~G), and were >100(range: 100~137) in the impacted sites, which indicates a poor condition (P). Underthe circumstances, organ tissues, such as the liver, kidney, and gills were largelyimpaired, so that efficient water quality managements are required in the outflowarea of the abandoned mine watershed.


1.Adams, S.M., A.M. Brown and R.W. Goede. 1993. Aquantitative health assessment index for rapid evaluation of fish condition in the field. Transactions of the American Fisheries Society 122: 63-73.
2.An, K.G. and J.H. Kim. 2005. A diagnosis of ecological health using a physical habitat assessment and multimetric fish model in Daejeon stream. Korean Journal of Limnology 38: 361-371.
3.An, K.G., J.Y. Lee, D.Y. Bae, J.H. Kim, S.J. Hwang, D.H. Won, J.K. Lee and C.S. Kim. 2006. Ecological assessments of aquatic environment using multi-metric model in major nationwide stream watersheds. Journal of Korean Society on Water Quality 22: 796-804.
4.An, K.G., S.S. Park and J.Y. Shin. 2002. An evaluation of a river health using the index of biological integrity along with relations to chemical and habitat conditions. Environment International 28: 411-420.
5.Anastasiadou, K. and E. Gidarakos. 2007. Toxicity evaluation for the broad area of the asbestos mine of northern Greece. Journal of Hazardous Materials 139: 9-18.
6.APHA (American Public Health Association). 2005. Standard methods for the examination of water and waste water, 21st ed. New York, NY: American Public Health Association.
7.Bae, D.Y., H.K. Kumara, J.H. Han, J.Y. Kim, K.W. Kim, Y.H. Kwon and K.G. An. 2010. Integrative ecological health assessments of an acid mine stream and in situ pilot tests for wastewater treatments. Ecological Engineering 36: 653-663.
8.Barbour, M.T., J. Gerritsen, B.D. Snyder and J.B. Stribling. 1999. Rapid bioassessment protocols for use in streams and eadeable rivers: periphyton, benthic wacroinverte- 84 Environmental Monitoring and Assessment (2007) 129: 79-85b rates and Fish, 2nd ed. EPA-841-B-99-002. Washington, DC: U.S. Environmental Protection Agency, Office of Water.
9.Bell, F.G. and L.J. Donnelly. 2006. Mining and its impact on the environment. Taylor and Francis, New York.
10.Cooper, E.L. and C.C. Wagner. 1973. The effects of acid mine drainage on fish populations, 114 pp. In: Fish, Food Organisms in Acid Mine Waters of Pennsylvania, Environmental Protection Agency Report, No., EPA-R3-73- 032.
11.Feasby, G. and R.K. Jones. 1994. Report of results of a workshop on mine reclamation-Toronto, Ontario, 10-11 March, 1994. Hosted by the IGWG-Industry Task Force on Mine Reclamation.
12.Gadgil, A. 1998. Drinking water in developing countries. Annual Review of Energy and the Environment 23: 253- 286.
13.Gerhardt, A., L. Janssens de Bisthoven and A.M.V.M. Soaresa. 2004. Macroinvertebrate response to acid mine drainage: Community metrics and on-line behavioural toxicity bioassay. Environmental Pollution 130: 263-274.
14.Gerhardt, A., L. Janssens de Bisthoven, Z. Mo, C. Wang and Z. Wang. 2002. Short-term behavioural responses of Orizias latipes (Pisces) and Macrobrachium nipponense (Crustacea) to municipal and pharmaceutical waste water in Beijing, China. Chemosphere-Section Environmental Toxicology and Risk Assessment 47: 35-47.
15.Gray, N.F. 1998. Acid mine drainage composition and the implications for its impact on lotic systems. Water Research 32: 2122-2134.
16.Judy, R.D., Jr. P.N. Seeley, T.M. Murray, S.C. Svirsky, M.R. Whitworth and L.S. Ischinger. 1984. National fisheries survey. Volume 1. Technical Report: initial findings. United States Fish and Wildlife Service. FWS/OBS-84/06.
17.Jung, H.B., S.T. Yun, S.O. Kim, C.S. So and M.C. Jung. 2003. Heavy metal contamination and the roles of retention pond and hydrologic mixing for removal of heavy metals in mine drainage, Kwangyang Au-Ag mine area. The Journal of Engineering Geology 13: 29-50.
18.Jung, M.C., M.Y. Jung and Y.W. Choi. 2004. Environmental assessment of heavy metals around abandoned Metalliferous mine in Korea. Korea Society of Economic and Environmental Geology 37: 21-33.
19.Karr, J.R. 1981. Assessment of biotic integrity using fish communities. Fishieries 6: 21-27.
20.Karr, J.R. and E.W. Chu. 2000. Sustaining living waters. Hydrobiologia 422: 1-14.
21.Karr, J.R., K.D. Fausch, P.L. Angermeier, P.R. Yant and I.J. Schlosse. 1986. Assessing biological integrity in running water: A method and its rationale, p. 28. In: Illinois national History Survey, Special Publication 5, Champaign, IL.
22.Kelly, M. 1991. Mining and the freshwater environment, Elsevier Science Publishers LTD, London.
23.Kim, J.Y., B.T. Lee, K.H. Shin, K.Y. Lee, K.W. Kim, K.G. An, Y.S. Park, J.Y. Kim and Y.H. Kwon. 2007b. Ecological health assessment and remediation of the stream impacted by acid mine drainage of the Gwangyang mine area. Environmental Monitoring Assessment 129: 79-85.
24.Kim, K.T., B.C. Lee, D.W. Kim and S.D. Kim. 2007a. Ecological risk assessment (ERA) of abandoned mine drainage (AMD) in Korea based on Vibrio fisheri, Selenastrum capricornutum, and Daphnia magna. Journal of Korean Society of Environmental Engineers 29: 163-168.
25.Kim, M.H., Y.S. Sho, E.J. Kim, S.Y. Chung and M.K. Hong. 2002. Studies on heavy metal contamination of agricultural products, soils and irrigation waters in abandoned mines. Journal of Korean Society of Food Hygiene and Safety 17: 178-182.
26.Kivaisi, A.K. 2001. The potential for constructed wetlands for wastewater treatment and reuse in developing countries: A review. Ecological Engineering 16: 545-560.
27.Lee, H.J., H.J. Kim, I.J. Oh, K.J. Cho, J.G. Kim and J.H. Jung. 2007. Assessment of heavy metal contamination and biological toxicity of mine drainages and sediments from abandoned mines. Journal of Korean Society on Water Quality 23: 287-293.
28.Lee, J.S., Y.N. Kim and K.H. Kim. 2010. Suitability assessment for agriculture of soils adjacent to abandoned mining areas using different human risk assessment models. The Journal of the Korean Society of Soil Science and Fertilizer 43: 552-561.
29.Lim, H.S., J.S. Lee, H.T. Chon and M. Sager. 2008. Heavy metal accumulation and health risk assessment in the vicinity of the abandoned Songcheon Au-Ag mine in Korea. Journal of Geochemical Exploration 96: 223-230.
30.Lottermoser, B.G. 2007. Mine wastes: Characterization, treatment and environmental impacts, 2nd ed. Springer, New York, p. 304.
31.MAC(Mining Association of Canada). 2002. Environmental progress report. Available from Ottawa, Ontario.
32.Maltby, L., S.A. Clayton, H. Yu, N. McLoughlin, R.W. Wood and D. Yin. 2000. Using single-species toxicity tests, community-level responses, and toxicity identification evaluations to investigate effluent impacts. Environmental Toxicology and Chemistry 19: 151-157.
33.Nelson, J.S. 1994. Fishes of the world (3th ed.). John Wiley & Sons, New York.
34.Nelson, M., H.T. Odum, M.T. Brown and A. Alling. 2001. Living of the land: Resource efficiency of wetland wastewater treatment. Advances in Space Research 27: 1547- 1556.
35.Ohio EPA. 1989. Biological criteria for the protection of aquatic life. Standardized biological field sampling and laboratory method for assessing fish and macroinvertebrate communities, vol. III. Ohio EPA Division of Water Quality Monitoring and Assessment Surface Water Section, Columbus, OH.
36.Plafkin, J.L., M.T. Barbour, K.D. Porter, S.K. Gross and R.M. Hughes. 1989. Rapid assessment protocols for use in streams and rivers: Benthic macroinvertebrate and fish, EPA/444/4-89-001, Office of Water Regulations and Standards, U.S. EPA, Washington, DC, USA.
37.Salomons, W. 1995. Environmental impact of metals derived from mining activities: Processes, predictions, prevention. Journal of Geochemical Exploration 52: 5-23.
38.Santore, R.C., D.M. Di Toro, P.R. Paquin, H.E. Allen and J.S. Meyer. 2001. Biotic ligand model of the acute toxicity of metals. 2. Application to acute copper toxicity in freshwater fish and Daphnia. Environmtal Toxicology Chemistry 20: 2397-2402.
39.Schmitt, C.J., W.G. Brumbaugh and T.W. May. 2007. Accumulation of metals in fish from lead-zinc mining areas of southeastern Missouri, USA. Ecotoxicology and Environmental Safety 67: 14-30.
40.So, C.S., S.T. Yun and S.H. Kwon. 1999. Gold-Silver mineralization of the Jungheung and Okdong mines, Korea: mineralogical and geochemical change in a cooling hydrothermal system. Neues Jahrbuch Fur Mineralogie-Abhandlungen 174: 223-248.
41.Strosnider, W.H. and R.W. Nairn. 2010. Effective passive treatment of high-strength acid mine drainage and raw municipal wastewater in Potosí, Bolivia using simple mutual incubations and limestone. Journal of Geochemical Exploration 105: 34-42.
42.U.S. EPA. 1991. Technical support document for water quality- based toxic control. EPA-505-2-90-001. U.S. EPA, Office of Water, Washington, D.C., USA.
43.U.S. EPA. 1993. Fish field and laboratory methods for evaluating the biological integrity of surface waters. EPA- 600-R-92-111. Environmental Monitoring systems Laboratory- Cincinnati office of Modeling, Monitoring systems, and quality assurance Office of Research Development, U.S. EPA, Cincinnati, Ohio 45268.